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human dna methylation microarray  (Agilent technologies)


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    Agilent technologies human dna methylation microarray
    Human Dna Methylation Microarray, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+dna+methylation+microarray/pmc09815458-117-8-16
    Average 90 stars, based on 1 article reviews
    human dna methylation microarray - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Genome Wide:

    Article Title: Genome-Wide Methylation Profiling of Schizophrenia
    Article Snippet: .. Genome-wide DNA methylation was assessed using the Agilent Human DNA Methylation Microarray (Agilent Technologies, Santa Clara, CA, USA) platform. ..

    Article Title: Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment.
    Article Snippet: Schizophrenia Research 232 (2021) 112–124 Contents lists available at ScienceDirect Schizophrenia Research j ourna l homepage: www.e lsev ie r .com/ locate /schres Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment Amanda J. Lisoway a,b,1, Cheng C. Chen a,b,1, Clement C. Zai a,b,c,d, Arun K. Tiwari a,d,2, James L. Kennedy a,b,d,⁎,2 a Molecular Brain Science Department, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada b Institute of Medical Science, University of Toronto, Canada c Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada d Department of Psychiatry, University of Toronto, Canada ⁎ Corresponding author at: Molecular Brain Science De and Mental Health, 250 College Street, Toronto, ON M5T 1 E-mail address: jim.kennedy@camh.ca (J.L.. Kennedy).. 1 Co-first authors.

    DNA Methylation Assay:

    Article Title: Genome-Wide Methylation Profiling of Schizophrenia
    Article Snippet: .. Genome-wide DNA methylation was assessed using the Agilent Human DNA Methylation Microarray (Agilent Technologies, Santa Clara, CA, USA) platform. ..

    Article Title: DNA methylation and antipsychotic treatment mechanisms in schizophrenia: Progress and future directions.
    Article Snippet: Antipsychotic response in schizophrenia is a complex, multifactorial trait influenced by pharmacogenetic factors.. With genetic studies thus far providing little biological insight or clinical utility, the field of pharmacoepigenomics has emerged to tackle the so-called “missing heritability” of drug response in disease.. Research on psychiatric disorders has only recently started to assess the link between epigenetic alterations and treatment outcomes.

    Article Title: The CpG island methylator phenotype in breast cancer is associated with the lobular subtype.
    Article Snippet: 2015 Background: Aberrations in DNA methylation patterns are well-described in human malignancies.. However, the existence of the ‘CpG island methylator phenotype’ (CIMP) in human breast cancer is still controversial.. Materials & methods: Illumina’s HumanMethylation 450K BeadChip was used to analyze genome-wide DNA methylation patterns.

    Article Title: Genome-Wide Analysis of DNA Methylation and Antituberculosis Drug-Induced Liver Injury in the Han Chinese Population.
    Article Snippet: Tuberculosis (TB) is one of the most prevalent infections.. However, anti-TB drugs induce adverse liver injury in up to 40% of patients.. Studies on candidate genes have suggested that single-nucleotide polymorphisms account for only a small contribution to the occurrence of anti-tuberculosis drug-induced liver injury (ATLI).

    Article Title: Methylome-wide association study of different responses to risperidone in schizophrenia
    Article Snippet: Following the instructions on the QIAamp DNA Blood Kits (Qiagen, United States of America), genomic DNA was obtained from whole blood samples. .. The methylation status was evaluated using the Agilent Human DNA Methylation Microarray (1 × 244 k; Agilent Technologies, Santa Clara, CA) according to the manufacturer’s recommendations. ..

    Article Title: Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment.
    Article Snippet: Schizophrenia Research 232 (2021) 112–124 Contents lists available at ScienceDirect Schizophrenia Research j ourna l homepage: www.e lsev ie r .com/ locate /schres Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment Amanda J. Lisoway a,b,1, Cheng C. Chen a,b,1, Clement C. Zai a,b,c,d, Arun K. Tiwari a,d,2, James L. Kennedy a,b,d,⁎,2 a Molecular Brain Science Department, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada b Institute of Medical Science, University of Toronto, Canada c Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada d Department of Psychiatry, University of Toronto, Canada ⁎ Corresponding author at: Molecular Brain Science De and Mental Health, 250 College Street, Toronto, ON M5T 1 E-mail address: jim.kennedy@camh.ca (J.L.. Kennedy).. 1 Co-first authors.

    Article Title: Global DNA methylation profiling uncovers distinct methylation patterns of protocadherin alpha4 in metastatic and non-metastatic rhabdomyosarcoma
    Article Snippet: .. DNA methylation profiling was carried out in RMS tumor samples using the Human DNA Methylation Microarray (Agilent Technologies, Santa Clara, CA, USA) consisting of about 244,000 (60-mer) probes designed to interrogate about 27,000 known CpG islands. .. The control genomic DNA and methylated dsDNA were labeled with Cy3 and Cy5 dye respectively using Agilent Genomic DNA labeling kit PLUS (Agilent Technologies, Santa Clara, CA, USA) and competitively hybridized to Human DNA Methylation microarrays platforms (GEO ID: GPL10878).

    Article Title: DNA methylation microarrays identify epigenetically regulated lipid related genes in obese patients with hypercholesterolemia
    Article Snippet: .. Human DNA Methylation Microarray (from Agilent Technologies) containing 27,627 probes for CpG islands was used for screening of DNA methylation status in 10 selected samples. ..

    Microarray:

    Article Title: Genome-Wide Methylation Profiling of Schizophrenia
    Article Snippet: .. Genome-wide DNA methylation was assessed using the Agilent Human DNA Methylation Microarray (Agilent Technologies, Santa Clara, CA, USA) platform. ..

    Article Title: DNA methylation and antipsychotic treatment mechanisms in schizophrenia: Progress and future directions.
    Article Snippet: Antipsychotic response in schizophrenia is a complex, multifactorial trait influenced by pharmacogenetic factors.. With genetic studies thus far providing little biological insight or clinical utility, the field of pharmacoepigenomics has emerged to tackle the so-called “missing heritability” of drug response in disease.. Research on psychiatric disorders has only recently started to assess the link between epigenetic alterations and treatment outcomes.

    Article Title: The CpG island methylator phenotype in breast cancer is associated with the lobular subtype.
    Article Snippet: 2015 Background: Aberrations in DNA methylation patterns are well-described in human malignancies.. However, the existence of the ‘CpG island methylator phenotype’ (CIMP) in human breast cancer is still controversial.. Materials & methods: Illumina’s HumanMethylation 450K BeadChip was used to analyze genome-wide DNA methylation patterns.

    Article Title: Genome-Wide Analysis of DNA Methylation and Antituberculosis Drug-Induced Liver Injury in the Han Chinese Population.
    Article Snippet: Tuberculosis (TB) is one of the most prevalent infections.. However, anti-TB drugs induce adverse liver injury in up to 40% of patients.. Studies on candidate genes have suggested that single-nucleotide polymorphisms account for only a small contribution to the occurrence of anti-tuberculosis drug-induced liver injury (ATLI).

    Article Title: Methylome-wide association study of different responses to risperidone in schizophrenia
    Article Snippet: Following the instructions on the QIAamp DNA Blood Kits (Qiagen, United States of America), genomic DNA was obtained from whole blood samples. .. The methylation status was evaluated using the Agilent Human DNA Methylation Microarray (1 × 244 k; Agilent Technologies, Santa Clara, CA) according to the manufacturer’s recommendations. ..

    Article Title: Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment.
    Article Snippet: Schizophrenia Research 232 (2021) 112–124 Contents lists available at ScienceDirect Schizophrenia Research j ourna l homepage: www.e lsev ie r .com/ locate /schres Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment Amanda J. Lisoway a,b,1, Cheng C. Chen a,b,1, Clement C. Zai a,b,c,d, Arun K. Tiwari a,d,2, James L. Kennedy a,b,d,⁎,2 a Molecular Brain Science Department, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada b Institute of Medical Science, University of Toronto, Canada c Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada d Department of Psychiatry, University of Toronto, Canada ⁎ Corresponding author at: Molecular Brain Science De and Mental Health, 250 College Street, Toronto, ON M5T 1 E-mail address: jim.kennedy@camh.ca (J.L.. Kennedy).. 1 Co-first authors.

    Article Title: Global DNA methylation profiling uncovers distinct methylation patterns of protocadherin alpha4 in metastatic and non-metastatic rhabdomyosarcoma
    Article Snippet: .. DNA methylation profiling was carried out in RMS tumor samples using the Human DNA Methylation Microarray (Agilent Technologies, Santa Clara, CA, USA) consisting of about 244,000 (60-mer) probes designed to interrogate about 27,000 known CpG islands. .. The control genomic DNA and methylated dsDNA were labeled with Cy3 and Cy5 dye respectively using Agilent Genomic DNA labeling kit PLUS (Agilent Technologies, Santa Clara, CA, USA) and competitively hybridized to Human DNA Methylation microarrays platforms (GEO ID: GPL10878).

    Article Title: DNA methylation microarrays identify epigenetically regulated lipid related genes in obese patients with hypercholesterolemia
    Article Snippet: .. Human DNA Methylation Microarray (from Agilent Technologies) containing 27,627 probes for CpG islands was used for screening of DNA methylation status in 10 selected samples. ..

    Methylation:

    Article Title: Genome-Wide Analysis of DNA Methylation and Antituberculosis Drug-Induced Liver Injury in the Han Chinese Population.
    Article Snippet: Tuberculosis (TB) is one of the most prevalent infections.. However, anti-TB drugs induce adverse liver injury in up to 40% of patients.. Studies on candidate genes have suggested that single-nucleotide polymorphisms account for only a small contribution to the occurrence of anti-tuberculosis drug-induced liver injury (ATLI).

    Article Title: Methylome-wide association study of different responses to risperidone in schizophrenia
    Article Snippet: Following the instructions on the QIAamp DNA Blood Kits (Qiagen, United States of America), genomic DNA was obtained from whole blood samples. .. The methylation status was evaluated using the Agilent Human DNA Methylation Microarray (1 × 244 k; Agilent Technologies, Santa Clara, CA) according to the manufacturer’s recommendations. ..

    Article Title: Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment.
    Article Snippet: Schizophrenia Research 232 (2021) 112–124 Contents lists available at ScienceDirect Schizophrenia Research j ourna l homepage: www.e lsev ie r .com/ locate /schres Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment Amanda J. Lisoway a,b,1, Cheng C. Chen a,b,1, Clement C. Zai a,b,c,d, Arun K. Tiwari a,d,2, James L. Kennedy a,b,d,⁎,2 a Molecular Brain Science Department, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada b Institute of Medical Science, University of Toronto, Canada c Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada d Department of Psychiatry, University of Toronto, Canada ⁎ Corresponding author at: Molecular Brain Science De and Mental Health, 250 College Street, Toronto, ON M5T 1 E-mail address: jim.kennedy@camh.ca (J.L.. Kennedy).. 1 Co-first authors.

    Immunoprecipitation:

    Article Title: Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment.
    Article Snippet: Schizophrenia Research 232 (2021) 112–124 Contents lists available at ScienceDirect Schizophrenia Research j ourna l homepage: www.e lsev ie r .com/ locate /schres Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment Amanda J. Lisoway a,b,1, Cheng C. Chen a,b,1, Clement C. Zai a,b,c,d, Arun K. Tiwari a,d,2, James L. Kennedy a,b,d,⁎,2 a Molecular Brain Science Department, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada b Institute of Medical Science, University of Toronto, Canada c Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada d Department of Psychiatry, University of Toronto, Canada ⁎ Corresponding author at: Molecular Brain Science De and Mental Health, 250 College Street, Toronto, ON M5T 1 E-mail address: jim.kennedy@camh.ca (J.L.. Kennedy).. 1 Co-first authors.

    Chromatin Immunoprecipitation:

    Article Title: Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment.
    Article Snippet: Schizophrenia Research 232 (2021) 112–124 Contents lists available at ScienceDirect Schizophrenia Research j ourna l homepage: www.e lsev ie r .com/ locate /schres Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment Amanda J. Lisoway a,b,1, Cheng C. Chen a,b,1, Clement C. Zai a,b,c,d, Arun K. Tiwari a,d,2, James L. Kennedy a,b,d,⁎,2 a Molecular Brain Science Department, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada b Institute of Medical Science, University of Toronto, Canada c Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada d Department of Psychiatry, University of Toronto, Canada ⁎ Corresponding author at: Molecular Brain Science De and Mental Health, 250 College Street, Toronto, ON M5T 1 E-mail address: jim.kennedy@camh.ca (J.L.. Kennedy).. 1 Co-first authors.

    Methylated DNA Immunoprecipitation:

    Article Title: Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment.
    Article Snippet: Schizophrenia Research 232 (2021) 112–124 Contents lists available at ScienceDirect Schizophrenia Research j ourna l homepage: www.e lsev ie r .com/ locate /schres Toward personalized medicine in schizophrenia: Genetics and epigenetics of antipsychotic treatment Amanda J. Lisoway a,b,1, Cheng C. Chen a,b,1, Clement C. Zai a,b,c,d, Arun K. Tiwari a,d,2, James L. Kennedy a,b,d,⁎,2 a Molecular Brain Science Department, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Toronto, ON, Canada b Institute of Medical Science, University of Toronto, Canada c Department of Laboratory Medicine and Pathobiology, University of Toronto, Canada d Department of Psychiatry, University of Toronto, Canada ⁎ Corresponding author at: Molecular Brain Science De and Mental Health, 250 College Street, Toronto, ON M5T 1 E-mail address: jim.kennedy@camh.ca (J.L.. Kennedy).. 1 Co-first authors.



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    TFCP2 binds to the core TF-binding region of CPEB1 when it is hypermethylated. a Silver staining of the nucleoproteins identified in <t>DNA</t> pull-down assay under various conditions. p-WT, the non-methylated CPEB1 promoter; p-Me, the hypermethylated CPEB1 promoter; control, magnetic beads without probes; Input, total nucleoproteins extracted from HCT116 cells; M, protein molecular mass marker. b WB detecting immunoreactive CEBPB in the nucleoprotein fraction after DNA pull-down with the anti-CEBPB antibody. The molecular mass of CEBPB is approximately 35 kDa. c EMSA revealed that CEBPB protein was unable to bind to its target sequence in the hypermethylated TF-binding region of CPEB1 ; 50 × cold probe WT, 50-fold concentration of the unlabelled wild-type CPEB1 promoter which was served as the competitor probe; Bio-Probe WT, a biotin-labelled wild-type probe of CPEB1 upstream region; Bio-Probe Mut, a biotin-labelled mutant probe of CPEB1 upstream region; Nucleoprotein, nucleoprotein extracted from HCT116 cells; Me-Bio Probe WT, a biotin-labelled hypermethylated probe of CPEB1 upstream region. d TFCP2 may be a candidate <t>methylation</t> reader at the upstream region of CPEB1 ; Methylation, the hypermethylated CPEB1 upstream region probe; Wild-type, the wild-type CPEB1 upstream region probe; Control, a probe with a scrambled sequence of CPEB1 upstream region; TF, the TFs capable of binding to the CPEB1 upstream as determined by ChIP-Seq. e Competitive EMSA to confirm TFCP2 as a methylation reader for CPEB1 . Bio-probe, a biotin-labelled wild-type CPEB1 upstream region probe; Me-Bio Probe, a biotin-labelled hypermethylated CPEB1 upstream region probe; 50 × Cold Probe, 50-fold concentration of the unlabelled wild-type CPEB1 upstream region probe that served as a competitor of the Bio-probe; 50 × Cold Me-Probe, 50-fold concentration the unlabelled hypermethylated CPEB1 upstream region probe that served as a competitor of the Me-Bio Probe
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    The culture and activation process of ovarian tissue in vitro did not cause abnormal <t>DNA</t> <t>methylation.</t> (A) No significant differences in total genome DNA methylation between three groups of human ovarian tissues were observed: normal group A (A1, A2, A3) (normal human ovarian fragment), control group B (B1, B2, B3) (treated with culture media only), and activated group C (C1, C2, C3) (treated with 10 μM activator MHY1485). The abscissa indicates the nine sample names, and the ordinate represents the methylation level (standardized beta value). (B) The PCA chart indicates the similarity between the samples by the method of decreasing dimensions. The three groups did not cluster together, indicating that the similarity of the samples within the same group was poor, and there were no significant differences between the three groups. | Delta Beta| ≥ 0.12 ( P < 0.01) is used to screen out the different sites, Delta Beta ≥ 0.12 indicates that the degree of methylation is increased, and Delta Beta ≤ –0.12 indicates that the degree of methylation is decreased. Three volcano plots between groups show the differential of the methylation sites.
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    Image Search Results


    Structure of the Biomarker References and Online Resources Provided

    Journal: Journal of Translational Medicine

    Article Title: SITC/iSBTc Cancer Immunotherapy Biomarkers Resource Document: Online resources and useful tools - a compass in the land of biomarker discovery

    doi: 10.1186/1479-5876-9-155

    Figure Lengend Snippet: Structure of the Biomarker References and Online Resources Provided

    Article Snippet: Examples Name: Agilent Human DNA Methylation Microarrays Comment: The array is specifically designed for analysis of methylated DNA derived from affinity-based isolation methods such as methylated DNA immunoprecipitation (MDIP) Website: http://www.genomics.agilent.com/CollectionSubpage.aspx?PageType=Product&SubPageType=ProductDetail&PageID=2157 Name: Nimblegen 2.1 M Whole-Genome Tiling Set Comment: Whole-genome formats are available in two versions: a 10-array set at 100 bp probe interval or a 4-array set at > 200 bp probe interval Website: http://www.nimblegen.com/products/methylation/whole_genome.html Name: Illumina 450 K Infinium Methylation BeadChip Kit Comment: This assay allow to interrogate > 450,000 methylation sites per sample at single-nucleotide resolution Website: http://www.illumina.com/products/methylation_450_beadchip_kits.ilmn

    Techniques: Biomarker Assay, Expressing, High Throughput Screening Assay, Hybridization, Methylation, Chromatin Immunoprecipitation, Next-Generation Sequencing, Sequencing, Software, Binding Assay

    TFCP2 binds to the core TF-binding region of CPEB1 when it is hypermethylated. a Silver staining of the nucleoproteins identified in DNA pull-down assay under various conditions. p-WT, the non-methylated CPEB1 promoter; p-Me, the hypermethylated CPEB1 promoter; control, magnetic beads without probes; Input, total nucleoproteins extracted from HCT116 cells; M, protein molecular mass marker. b WB detecting immunoreactive CEBPB in the nucleoprotein fraction after DNA pull-down with the anti-CEBPB antibody. The molecular mass of CEBPB is approximately 35 kDa. c EMSA revealed that CEBPB protein was unable to bind to its target sequence in the hypermethylated TF-binding region of CPEB1 ; 50 × cold probe WT, 50-fold concentration of the unlabelled wild-type CPEB1 promoter which was served as the competitor probe; Bio-Probe WT, a biotin-labelled wild-type probe of CPEB1 upstream region; Bio-Probe Mut, a biotin-labelled mutant probe of CPEB1 upstream region; Nucleoprotein, nucleoprotein extracted from HCT116 cells; Me-Bio Probe WT, a biotin-labelled hypermethylated probe of CPEB1 upstream region. d TFCP2 may be a candidate methylation reader at the upstream region of CPEB1 ; Methylation, the hypermethylated CPEB1 upstream region probe; Wild-type, the wild-type CPEB1 upstream region probe; Control, a probe with a scrambled sequence of CPEB1 upstream region; TF, the TFs capable of binding to the CPEB1 upstream as determined by ChIP-Seq. e Competitive EMSA to confirm TFCP2 as a methylation reader for CPEB1 . Bio-probe, a biotin-labelled wild-type CPEB1 upstream region probe; Me-Bio Probe, a biotin-labelled hypermethylated CPEB1 upstream region probe; 50 × Cold Probe, 50-fold concentration of the unlabelled wild-type CPEB1 upstream region probe that served as a competitor of the Bio-probe; 50 × Cold Me-Probe, 50-fold concentration the unlabelled hypermethylated CPEB1 upstream region probe that served as a competitor of the Me-Bio Probe

    Journal: Clinical Epigenetics

    Article Title: DNA hypermethylation contributes to colorectal cancer metastasis by regulating the binding of CEBPB and TFCP2 to the CPEB1 promoter

    doi: 10.1186/s13148-021-01071-z

    Figure Lengend Snippet: TFCP2 binds to the core TF-binding region of CPEB1 when it is hypermethylated. a Silver staining of the nucleoproteins identified in DNA pull-down assay under various conditions. p-WT, the non-methylated CPEB1 promoter; p-Me, the hypermethylated CPEB1 promoter; control, magnetic beads without probes; Input, total nucleoproteins extracted from HCT116 cells; M, protein molecular mass marker. b WB detecting immunoreactive CEBPB in the nucleoprotein fraction after DNA pull-down with the anti-CEBPB antibody. The molecular mass of CEBPB is approximately 35 kDa. c EMSA revealed that CEBPB protein was unable to bind to its target sequence in the hypermethylated TF-binding region of CPEB1 ; 50 × cold probe WT, 50-fold concentration of the unlabelled wild-type CPEB1 promoter which was served as the competitor probe; Bio-Probe WT, a biotin-labelled wild-type probe of CPEB1 upstream region; Bio-Probe Mut, a biotin-labelled mutant probe of CPEB1 upstream region; Nucleoprotein, nucleoprotein extracted from HCT116 cells; Me-Bio Probe WT, a biotin-labelled hypermethylated probe of CPEB1 upstream region. d TFCP2 may be a candidate methylation reader at the upstream region of CPEB1 ; Methylation, the hypermethylated CPEB1 upstream region probe; Wild-type, the wild-type CPEB1 upstream region probe; Control, a probe with a scrambled sequence of CPEB1 upstream region; TF, the TFs capable of binding to the CPEB1 upstream as determined by ChIP-Seq. e Competitive EMSA to confirm TFCP2 as a methylation reader for CPEB1 . Bio-probe, a biotin-labelled wild-type CPEB1 upstream region probe; Me-Bio Probe, a biotin-labelled hypermethylated CPEB1 upstream region probe; 50 × Cold Probe, 50-fold concentration of the unlabelled wild-type CPEB1 upstream region probe that served as a competitor of the Bio-probe; 50 × Cold Me-Probe, 50-fold concentration the unlabelled hypermethylated CPEB1 upstream region probe that served as a competitor of the Me-Bio Probe

    Article Snippet: Publicly available high-throughput DNA methylation microarray data (Illumina Human Methylation 450 K) of 387 CRC tumours and 45 samples of para-tumour tissue were obtained from the TCGA database (level-3).

    Techniques: Binding Assay, Silver Staining, Pull Down Assay, Methylation, Control, Magnetic Beads, Marker, Sequencing, Concentration Assay, Mutagenesis, ChIP-sequencing

    The culture and activation process of ovarian tissue in vitro did not cause abnormal DNA methylation. (A) No significant differences in total genome DNA methylation between three groups of human ovarian tissues were observed: normal group A (A1, A2, A3) (normal human ovarian fragment), control group B (B1, B2, B3) (treated with culture media only), and activated group C (C1, C2, C3) (treated with 10 μM activator MHY1485). The abscissa indicates the nine sample names, and the ordinate represents the methylation level (standardized beta value). (B) The PCA chart indicates the similarity between the samples by the method of decreasing dimensions. The three groups did not cluster together, indicating that the similarity of the samples within the same group was poor, and there were no significant differences between the three groups. | Delta Beta| ≥ 0.12 ( P < 0.01) is used to screen out the different sites, Delta Beta ≥ 0.12 indicates that the degree of methylation is increased, and Delta Beta ≤ –0.12 indicates that the degree of methylation is decreased. Three volcano plots between groups show the differential of the methylation sites.

    Journal: Frontiers in Genetics

    Article Title: The Efficacy and Safety of the mTOR Signaling Pathway Activator, MHY1485, for in vitro Activation of Human Ovarian Tissue

    doi: 10.3389/fgene.2020.603683

    Figure Lengend Snippet: The culture and activation process of ovarian tissue in vitro did not cause abnormal DNA methylation. (A) No significant differences in total genome DNA methylation between three groups of human ovarian tissues were observed: normal group A (A1, A2, A3) (normal human ovarian fragment), control group B (B1, B2, B3) (treated with culture media only), and activated group C (C1, C2, C3) (treated with 10 μM activator MHY1485). The abscissa indicates the nine sample names, and the ordinate represents the methylation level (standardized beta value). (B) The PCA chart indicates the similarity between the samples by the method of decreasing dimensions. The three groups did not cluster together, indicating that the similarity of the samples within the same group was poor, and there were no significant differences between the three groups. | Delta Beta| ≥ 0.12 ( P < 0.01) is used to screen out the different sites, Delta Beta ≥ 0.12 indicates that the degree of methylation is increased, and Delta Beta ≤ –0.12 indicates that the degree of methylation is decreased. Three volcano plots between groups show the differential of the methylation sites.

    Article Snippet: We further used a human high-throughput DNA methylation microarray (Infinium Human Methylation EPIC BeadChip: 850k chip) to analyze the effects of the activator MHY1485 on the methylation status of the human genome in the ovary.

    Techniques: Activation Assay, In Vitro, DNA Methylation Assay, Control, Methylation